anti β adaptin Search Results


anti-δ-adaptin  (Becton Dickinson)
90
Becton Dickinson anti-δ-adaptin
Neuron-specific deletion of Scyl2. A, SCYL2 expression in the brain and liver extracts obtained from Scyl2+/+, NesCre+;Scyl2+/fl, and NesCre+;Scyl2fl/fl mice. Brain and liver extracts from Scyl2+/+, NesCre+;Scyl2+/fl, and NesCre+;Scyl2fl/fl mice were resolved by SDS-PAGE and analyzed by Western blotting, using antibodies against SCYL2 (α-SCYL2) and <t>μ2-adaptin</t> (AP50) as control. Note the absence of SCYL2 in the brain of NesCre+;Scyl2fl/fl mice but not the liver of the same mouse. B, Photograph of NesCre+;Scyl2+/fl and NesCre+;Scyl2fl/fl mice at birth. Note the absence of milk in the stomach of the NesCre+;Scyl2fl/fl mouse. Scale bar, 10 mm. C, Photograph of 6-week-old NesCre+;Scyl2+/fl and NesCre+;Scyl2fl/fl mice. NesCre+;Scyl2fl/fl mice exhibited growth retardation that was apparent by 2 weeks of age and remained throughout their lifetime. Scale bar, 10 mm. D, Abnormal reflexes of NesCre+;Scyl2fl/fl mice. When suspended by their tails, NesCre+;Scyl2+/fl mice spread their hind limbs, whereas NesCre+;Scyl2fl/fl mice grasped their hind limbs and forelimbs to their body. E, Grip strength in NesCre+;Scyl2fl/fl mice. The inverted grid test was performed on 10-week-old naive NesCre+;Scyl2+/fl (n = 7) and NesCre+;Scyl2fl/fl (n = 7) males as described in Materials and Methods. Control mice held on significantly longer on the inverted grid than NesCre+;Scyl2fl/fl mice. Data are expressed as the mean ± SEM. p values, determined by the two-tailed Student's t test, are indicated on the graph. During the tail suspension test, NesCre+;Scyl2+/fl mice explored the cage grid, but NesCre+;Scyl2fl/fl mice remained stationary. F–H, Depression-like behavior in NesCre+;Scyl2fl/fl mice. Depression-like behavior was determined in NesCre+;Scyl2+/fl and NesCre+;Scyl2fl/fl mice as described in Materials and Methods. Latency to immobility, a measure of depression-like behavior (F), duration of immobility (G), and strength of movement (H) were determined in NesCre+;Scyl2+/fl (n = 7) and NesCre+;Scyl2fl/fl (n = 7) animals. Data are mean ± SEM. p values, determined by the two-tailed Student's t test, are shown on the graph.
Anti δ Adaptin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B2+adaptin/anti+%CE%B2+adaptin/pmc04510291-118-44-46
Average 90 stars, based on 1 article reviews
anti-δ-adaptin - by Bioz Stars, 2026-11
90/100 stars
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goat anti-β-adaptin  (Becton Dickinson)
90
Becton Dickinson goat anti-β-adaptin
Neuron-specific deletion of Scyl2. A, SCYL2 expression in the brain and liver extracts obtained from Scyl2+/+, NesCre+;Scyl2+/fl, and NesCre+;Scyl2fl/fl mice. Brain and liver extracts from Scyl2+/+, NesCre+;Scyl2+/fl, and NesCre+;Scyl2fl/fl mice were resolved by SDS-PAGE and analyzed by Western blotting, using antibodies against SCYL2 (α-SCYL2) and <t>μ2-adaptin</t> (AP50) as control. Note the absence of SCYL2 in the brain of NesCre+;Scyl2fl/fl mice but not the liver of the same mouse. B, Photograph of NesCre+;Scyl2+/fl and NesCre+;Scyl2fl/fl mice at birth. Note the absence of milk in the stomach of the NesCre+;Scyl2fl/fl mouse. Scale bar, 10 mm. C, Photograph of 6-week-old NesCre+;Scyl2+/fl and NesCre+;Scyl2fl/fl mice. NesCre+;Scyl2fl/fl mice exhibited growth retardation that was apparent by 2 weeks of age and remained throughout their lifetime. Scale bar, 10 mm. D, Abnormal reflexes of NesCre+;Scyl2fl/fl mice. When suspended by their tails, NesCre+;Scyl2+/fl mice spread their hind limbs, whereas NesCre+;Scyl2fl/fl mice grasped their hind limbs and forelimbs to their body. E, Grip strength in NesCre+;Scyl2fl/fl mice. The inverted grid test was performed on 10-week-old naive NesCre+;Scyl2+/fl (n = 7) and NesCre+;Scyl2fl/fl (n = 7) males as described in Materials and Methods. Control mice held on significantly longer on the inverted grid than NesCre+;Scyl2fl/fl mice. Data are expressed as the mean ± SEM. p values, determined by the two-tailed Student's t test, are indicated on the graph. During the tail suspension test, NesCre+;Scyl2+/fl mice explored the cage grid, but NesCre+;Scyl2fl/fl mice remained stationary. F–H, Depression-like behavior in NesCre+;Scyl2fl/fl mice. Depression-like behavior was determined in NesCre+;Scyl2+/fl and NesCre+;Scyl2fl/fl mice as described in Materials and Methods. Latency to immobility, a measure of depression-like behavior (F), duration of immobility (G), and strength of movement (H) were determined in NesCre+;Scyl2+/fl (n = 7) and NesCre+;Scyl2fl/fl (n = 7) animals. Data are mean ± SEM. p values, determined by the two-tailed Student's t test, are shown on the graph.
Goat Anti β Adaptin, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+%CE%B2+adaptin/goat+anti+%CE%B2+adaptin/pm23902689-132-15-17
Average 90 stars, based on 1 article reviews
goat anti-β-adaptin - by Bioz Stars, 2026-11
90/100 stars
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rabbit anti-human beta-adaptin, internal  (RayBiotech inc)
N/A
beta-Adaptin antibody [N3C1], Internal, 0.55 mg/ml (100 µl)
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rabbit anti-human beta-adaptin, internal  (RayBiotech)
N/A
beta-Adaptin antibody [N3C1], Internal, 0.55 mg/ml (100 µl)
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Image Search Results


Neuron-specific deletion of Scyl2. A, SCYL2 expression in the brain and liver extracts obtained from Scyl2+/+, NesCre+;Scyl2+/fl, and NesCre+;Scyl2fl/fl mice. Brain and liver extracts from Scyl2+/+, NesCre+;Scyl2+/fl, and NesCre+;Scyl2fl/fl mice were resolved by SDS-PAGE and analyzed by Western blotting, using antibodies against SCYL2 (α-SCYL2) and μ2-adaptin (AP50) as control. Note the absence of SCYL2 in the brain of NesCre+;Scyl2fl/fl mice but not the liver of the same mouse. B, Photograph of NesCre+;Scyl2+/fl and NesCre+;Scyl2fl/fl mice at birth. Note the absence of milk in the stomach of the NesCre+;Scyl2fl/fl mouse. Scale bar, 10 mm. C, Photograph of 6-week-old NesCre+;Scyl2+/fl and NesCre+;Scyl2fl/fl mice. NesCre+;Scyl2fl/fl mice exhibited growth retardation that was apparent by 2 weeks of age and remained throughout their lifetime. Scale bar, 10 mm. D, Abnormal reflexes of NesCre+;Scyl2fl/fl mice. When suspended by their tails, NesCre+;Scyl2+/fl mice spread their hind limbs, whereas NesCre+;Scyl2fl/fl mice grasped their hind limbs and forelimbs to their body. E, Grip strength in NesCre+;Scyl2fl/fl mice. The inverted grid test was performed on 10-week-old naive NesCre+;Scyl2+/fl (n = 7) and NesCre+;Scyl2fl/fl (n = 7) males as described in Materials and Methods. Control mice held on significantly longer on the inverted grid than NesCre+;Scyl2fl/fl mice. Data are expressed as the mean ± SEM. p values, determined by the two-tailed Student's t test, are indicated on the graph. During the tail suspension test, NesCre+;Scyl2+/fl mice explored the cage grid, but NesCre+;Scyl2fl/fl mice remained stationary. F–H, Depression-like behavior in NesCre+;Scyl2fl/fl mice. Depression-like behavior was determined in NesCre+;Scyl2+/fl and NesCre+;Scyl2fl/fl mice as described in Materials and Methods. Latency to immobility, a measure of depression-like behavior (F), duration of immobility (G), and strength of movement (H) were determined in NesCre+;Scyl2+/fl (n = 7) and NesCre+;Scyl2fl/fl (n = 7) animals. Data are mean ± SEM. p values, determined by the two-tailed Student's t test, are shown on the graph.

Journal: The Journal of Neuroscience

Article Title: SCYL2 Protects CA3 Pyramidal Neurons from Excitotoxicity during Functional Maturation of the Mouse Hippocampus

doi: 10.1523/JNEUROSCI.2056-14.2015

Figure Lengend Snippet: Neuron-specific deletion of Scyl2. A, SCYL2 expression in the brain and liver extracts obtained from Scyl2+/+, NesCre+;Scyl2+/fl, and NesCre+;Scyl2fl/fl mice. Brain and liver extracts from Scyl2+/+, NesCre+;Scyl2+/fl, and NesCre+;Scyl2fl/fl mice were resolved by SDS-PAGE and analyzed by Western blotting, using antibodies against SCYL2 (α-SCYL2) and μ2-adaptin (AP50) as control. Note the absence of SCYL2 in the brain of NesCre+;Scyl2fl/fl mice but not the liver of the same mouse. B, Photograph of NesCre+;Scyl2+/fl and NesCre+;Scyl2fl/fl mice at birth. Note the absence of milk in the stomach of the NesCre+;Scyl2fl/fl mouse. Scale bar, 10 mm. C, Photograph of 6-week-old NesCre+;Scyl2+/fl and NesCre+;Scyl2fl/fl mice. NesCre+;Scyl2fl/fl mice exhibited growth retardation that was apparent by 2 weeks of age and remained throughout their lifetime. Scale bar, 10 mm. D, Abnormal reflexes of NesCre+;Scyl2fl/fl mice. When suspended by their tails, NesCre+;Scyl2+/fl mice spread their hind limbs, whereas NesCre+;Scyl2fl/fl mice grasped their hind limbs and forelimbs to their body. E, Grip strength in NesCre+;Scyl2fl/fl mice. The inverted grid test was performed on 10-week-old naive NesCre+;Scyl2+/fl (n = 7) and NesCre+;Scyl2fl/fl (n = 7) males as described in Materials and Methods. Control mice held on significantly longer on the inverted grid than NesCre+;Scyl2fl/fl mice. Data are expressed as the mean ± SEM. p values, determined by the two-tailed Student's t test, are indicated on the graph. During the tail suspension test, NesCre+;Scyl2+/fl mice explored the cage grid, but NesCre+;Scyl2fl/fl mice remained stationary. F–H, Depression-like behavior in NesCre+;Scyl2fl/fl mice. Depression-like behavior was determined in NesCre+;Scyl2+/fl and NesCre+;Scyl2fl/fl mice as described in Materials and Methods. Latency to immobility, a measure of depression-like behavior (F), duration of immobility (G), and strength of movement (H) were determined in NesCre+;Scyl2+/fl (n = 7) and NesCre+;Scyl2fl/fl (n = 7) animals. Data are mean ± SEM. p values, determined by the two-tailed Student's t test, are shown on the graph.

Article Snippet: The following antibodies were used: anti-clathrin heavy chain antibody (anti-CHC; BD Biosciences, 610500), anti-CHC antibody (X-22, Abcam, ab2731), anti-α-adaptin (BD Biosciences, 610502), anti-β2-adaptin (BD Biosciences, 610382), anti-γ-adaptin (BD Biosciences, 36120), anti-βNAP (β3-adaptin, BD Biosciences, 51-9002031), anti-p47 (μ3-adaptin, BD Biosciences, 51-9002064) anti-μ2-adaptin (AP50, BD Biosciences, 611351) anti-δ-adaptin (BD Biosciences, 51-9001906), anti-NMDA receptor 1 (NR1, Sigma, G8913), anti-NR2A (Sigma, G9038), anti-Grik4 (anti-KA1 receptor, Sigma, PRS4391), anti-GFAP (DAKO, Z0334), anti-c-Fos (Cell Signaling Technology, clone 9F6, 2250), anti-Iba1 (Wako, 019-19741), anti-cleaved-caspase 3 (Biocare, CP-229C), anti-BAX (Santa Cruz Biotechnology, sc-493), anti-amphiphysin (BD Biosciences, 51-9001902), anti-AP180 (BD Biosciences, 51-9001899), anti-Vti1b (BD Biosciences, 611405), anti-PSD-95 clone D74D3 (Cell Signaling Technology, 3409), anti-PSD-95 clone 6G6-1C9 (Pierce, MA1-045), anti-synaptophysin (Millipore, AB1543P), anti-MPR300 D3V8c (Cell Signaling Technology, 14364), anti-EGFR antibody (Fitzgerald, 20-ES04), anti-β-actin (Sigma A5441), anti-rabbit IgG HRP-linked antibody (Jackson ImmunoResearch Laboratories, 111-035-003), anti-mouse IgG HRP-linked antibody (Jackson ImmunoResearch Laboratories, 115-035-174), AlexaFluor-568 donkey anti-mouse IgG (H+L) (Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A10037","term_id":"489102","term_text":"A10037"}} A10037 ), AlexaFluor-488 goat anti-rabbit IgG (H+L) (Invitrogen, {"type":"entrez-nucleotide","attrs":{"text":"A11008","term_id":"492390","term_text":"A11008"}} A11008 ), and a biotinylated goat anti-rabbit antibody (Vector Laboratories, BA-1000).

Techniques: Expressing, SDS Page, Western Blot, Two Tailed Test